Anti-Neurofibromin antibody (ab70616)


  • Product nameAnti-Neurofibromin antibody
    See all Neurofibromin primary antibodies
  • Description
    Rabbit polyclonal to Neurofibromin
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    A synthetic peptide corresponding to the C terminus of human Neurofibromin.

  • Positive control
    • Whole cell lysates derived from hypoxic mouse brain.



Our Abpromise guarantee covers the use of ab70616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 240 kDa (predicted molecular weight: 319 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.


  • FunctionStimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
  • Involvement in diseaseDefects in NF1 are the cause of neurofibromatosis type 1 (NF1) [MIM:162200]; also known as von Recklinghausen syndrome. A disease characterized by patches of skin pigmentation (cafe-au-lait spots), Lisch nodules of the iris, tumors in the peripheral nervous system and fibromatous skin tumors. Individuals with the disorder have increased susceptibility to the development of benign and malignant tumors.
    Defects in NF1 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Germline mutations of NF1 account for the association of JMML with type 1 neurofibromatosis (NF1).
    Defects in NF1 are the cause of Watson syndrome (WS) [MIM:193520]. WS is characterized by the presence of pulmonary stenosis, cafe-au-lait spots, and mental retardation. WS is considered as an atypical form of NF1.
    Defects in NF1 are a cause of familial spinal neurofibromatosis (FSNF) [MIM:162210]. Familial spinal NF is considered to be an alternative form of neurofibromatosis, showing multiple spinal tumors.
    Defects in NF1 are a cause of neurofibromatosis-Noonan syndrome (NFNS) [MIM:601321]. NFNS is characterized by manifestations of both NF1 and Noonan syndrome (NS). NS is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis.
    Defects in NF1 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similaritiesContains 1 CRAL-TRIO domain.
    Contains 1 Ras-GAP domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686J1293 antibody
    • FLJ21220 antibody
    • Neurofibromatosis Noonan syndrome antibody
    • Neurofibromatosis related protein NF 1 antibody
    • Neurofibromatosis related protein NF1 antibody
    • neurofibromatosis type I antibody
    • Neurofibromatosis-related protein NF-1 antibody
    • Neurofibromin 1 antibody
    • Neurofibromin truncated antibody
    • Neurofibromin1 antibody
    • NF 1 antibody
    • NF antibody
    • NF1 antibody
    • NF1_HUMAN antibody
    • NFNS antibody
    • Type 1 Neurofibromatosis antibody
    • von Recklinghausen disease neurofibromin antibody
    • von Recklinghausen disease related protein VRNF antibody
    • VRNF antibody
    • WATS antibody
    • Watson disease related protein WSS antibody
    • Watson syndrome antibody
    • WSS antibody
    see all

Anti-Neurofibromin antibody images

References for Anti-Neurofibromin antibody (ab70616)

ab70616 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (zebrafish 3 day post fertilization whole embryos l)
Loading amount 50 µg
Specification zebrafish 3 day post fertilization whole embryos l
Gel Running Conditions Reduced Denaturing (3-8% TA Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted Nov 11 2009