The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa).
FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Sequence similaritiesBelongs to the intermediate filament family.
Post-translational modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
Immunocytochemistry/ Immunofluorescence - 200 kD Neurofilament Heavy antibody (ab72996)
Immunofluorescence of rat cortical neurons and glia using ab72996 at 1/25000 dilution showing Neurofilament heavy polypeptide staining (red).
References for Anti-Neurofilament heavy polypeptide antibody (ab72996)
This product has been referenced in:
Gershoni-Emek N et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics15:506-22 (2016).
Read more (PubMed: 26598648) »
Schrenk-Siemens K et al. PIEZO2 is required for mechanotransduction in human stem cell-derived touch receptors. Nat Neurosci18:10-6 (2015).
Read more (PubMed: 25469543) »