Anti-Neurofilament heavy polypeptide antibody (ab72996)


  • Product nameAnti-Neurofilament heavy polypeptide antibody
    See all Neurofilament heavy polypeptide primary antibodies
  • Description
    Chicken polyclonal to Neurofilament heavy polypeptide
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide.

  • Positive control
    • Rat cerebral cortex lysate. Rat cortical neurons and glia.



Our Abpromise guarantee covers the use of ab72996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa).
ICC/IF 1/25000.


  • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody images

  • Anti-Neurofilament heavy polypeptide antibody (ab72996) at 1/50000 dilution + Rat cerebral cortex lysate

    Predicted band size : 115 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of rat cortical neurons and glia using ab72996 at 1/25000 dilution showing Neurofilament heavy polypeptide staining (red).

References for Anti-Neurofilament heavy polypeptide antibody (ab72996)

This product has been referenced in:
  • Gershoni-Emek N  et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics 15:506-22 (2016). ICC/IF . Read more (PubMed: 26598648) »
  • Schrenk-Siemens K  et al. PIEZO2 is required for mechanotransduction in human stem cell-derived touch receptors. Nat Neurosci 18:10-6 (2015). Read more (PubMed: 25469543) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 7% · Temperature: 25°C
Sample Mouse Cell (DRG neurons)
Specification DRG neurons
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde

Mr. Shachar Dagan

Verified customer

Submitted Dec 16 2013