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Recombinant fragment corresponding to Cow Neurofilament heavy polypeptide (C terminal).
Our Abpromise guarantee covers the use of ab8135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19075249|
|ICC||Use at an assay dependent concentration.|
ab8135 staining Neurofilament heavy polypeptide in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections).
Tissue was fixed with formaldehyde, blocked with 5% serum for 1 hour at 25°C and permeabilzed with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) for 12 hours at 25°C. An Alexa Fluor® 555 goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.
Immunocytochemical staining of 33B cells (rat oligodendroglioma) with ab8135 at 1/1000. Cells were cultured in DMEM/10% FCS on coverslips in 12 well plates. Cells were washed with PBS and fixed in 4% PFA until staining. Cells were washed teice for 2 minutes and then treated with 3% hydrogen peroxide in methanol for 15 minutes at room temperature. The sample was blocked in 10% goat serum in 1% PBSA and then incubated with the primary antibody in 1% PBSA for 2 hours at room temperature. A biotinylated goat anti-rabbit was used as the secondary at 1/200 and then incubated with ABC for 30 minutes at room temperature, followed by DAB in PBS (brown). The sample was counter-stained with haematoxylin.
ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.
The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.
The primary antibody was incubated for 1 hour at 25°C.
Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.
Green = alpha bungarotoxin stained post-synaptic neuromuscular junction.
Red = ab8135 staining Neurofilament heavy polypeptide