Anti-Neurofilament heavy polypeptide antibody [NF-05] (ab118812)


  • Product name
    Anti-Neurofilament heavy polypeptide antibody [NF-05]
    See all Neurofilament heavy polypeptide primary antibodies
  • Description
    Mouse monoclonal [NF-05] to Neurofilament heavy polypeptide
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human Neurofilament heavy polypeptide. This antibody was raised against pig brain neurofilament protein-enriched fraction after depolymerization of microtubules.
    Database link: P12036

  • Positive control
    • Human Brain (Cerebellum and Cortex) and Peripheral Nerve tissues; Human brain homogenate lysate.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab118812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.


  • Function
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in disease
    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all


  • ab118812, at 10 µg/ml, staining Neurofilament heavy polypeptide in formalin-fixed, paraffin-embedded Human Brain (Cerebellum) tissue by Immunohistochemistry.

  • ab118812, at 10 µg/ml, staining Neurofilament heavy polypeptide in formalin-fixed, paraffin-embedded Human Brain (Cortex) tissue by Immunohistochemistry.

  • ab118812, at 10 µg/ml, staining Neurofilament heavy polypeptide in formalin-fixed, paraffin-embedded Human Peripheral Nerve tissue by Immunohistochemistry.


ab118812 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your email.
The size difference will effect WB not IHC. However it will more depend on the tissues section you will be using.

Please check the localization of different chains in axons

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Thank you for contacting us. I am afraid we do not have exact match of monoclonal the customer is looking for. We however have many antibodies that are against the neurofilament proteins that forms part of Axons. As by definition Neurofilaments (NFs) a...

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