Overview

  • Product name
    Anti-Neurogenin 1 antibody
    See all Neurogenin 1 primary antibodies
  • Description
    Mouse monoclonal to Neurogenin 1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Recombinant fragment: SEALLHSLRR SRRVKANDRE RNRMHNLNAA LDALRSVLPS FPDDTKLTKI ETLRFAYNYI WALAETLRLA DQGLPGGGAR ER, corresponding to amino acids 82-163 of human Neurogenin 1 (NP_006152) with a proprietary tag.

  • Positive control
    • WB: The immunoge; HeLa nuclear extract; Y-79 cell lysate ICC/IF: HeLa cell
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab89461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant, tagged Neurogenin 1 is approximately 10 ng/ml when used as a capture antibody.
ICC/IF Use a concentration of 50 µg/ml.

Target

Images

  • Anti-Neurogenin 1 antibody (ab89461) at 1 µg/ml + recombinant, tagged human Neurogenin 1 protein fragment (the immunogen) at 0.2 µg

    Predicted band size : 26 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-Neurogenin 1 antibody (ab89461) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-Neurogenin 1 antibody (ab89461) at 1 µg/ml + Y-79 cell lysate at 50 µg

    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • ab89461, at 50 µg/ml, staining Neurogenin 1 in HeLa cell by Immunofluorescence.

References

This product has been referenced in:
  • Jahan I  et al. Expression of neurog1 instead of atoh1 can partially rescue organ of corti cell survival. PLoS One 7:e30853 (2012). WB ; Mouse . Read more (PubMed: 22292060) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E11.5 mouse embryos transverse section)
Specification
E11.5 mouse embryos transverse section
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step
NOVOCASTRA protein block as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 13 2013

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

DISCOUNT CODES: 100%ABR-E7TFV for testing ab89461 in Zebrafish 100%ABR-E7TF3 for testing ab67168 in Zebrafish 100%ABR-E7TFM for testing ab101699 in Zebrafish Expiration date for all the three codes: 22. February 2012 I am very pleased to hear you would...

Read More

Thank you very much for your inquiry. Indeed, it is difficult to find antibodies crossreactive with Zebrafish. I don't know whether I have already told you about our list of Zebrafish crossreactive antibodies? It can be found under the following link: ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (SVZ Cell Culture)
Loading amount
40 µg
Specification
SVZ Cell Culture
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up