Anti-Neurogenin 2 antibody (ab52729)

Overview

  • Product nameAnti-Neurogenin 2 antibody
    See all Neurogenin 2 primary antibodies
  • Description
    Mouse polyclonal to Neurogenin 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fusion protein: TPMSSSADEE EDEELRRPGS ARGQRGAEAG QGVQGSPASG AGGCRPGRLL GLMHECKRRP SRSRAVSRGA KTAETVQRIK KTRRLKANNR ERNRMHNLNA, corresponding to amino acids 31-130 of Neurogenin 2

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa). This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionInvolved in neurogenesis.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Atoh 4 antibody
    • Atoh4 antibody
    • bHLHa8 antibody
    • Class A basic helix-loop-helix protein 8 antibody
    • Math 4A antibody
    • Math4A antibody
    • MGC antibody
    • MGC46562 antibody
    • NEUROG 2 antibody
    • NEUROG2 antibody
    • Neurogenin-2 antibody
    • Neurogenin2 antibody
    • NGN 2 antibody
    • NGN-2 antibody
    • NGN2 antibody
    • NGN2_HUMAN antibody
    • Protein atonal homolog 4 antibody
    see all

Anti-Neurogenin 2 antibody images

  • All lanes : Anti-Neurogenin 2 antibody (ab52729) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged recombinant fusion protein)

    Secondary
    Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 29 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-Neurogenin 2 antibody (ab52729)

ab52729 has not yet been referenced specifically in any publications.

Product Wall

I recommend the rabbit monoclonal antibody ab109236 for detection of rabbit neurogenin 2. Although we do not have experimental data, reactivity with the rabbit protein is likely since the immunogen amino acid sequence, derived from human neurogenin 2, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"