Anti-Neurogenin 2 antibody [EPR4177] (ab109236)

Overview

  • Product nameAnti-Neurogenin 2 antibody [EPR4177]
    See all Neurogenin 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4177] to Neurogenin 2
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Neurogenin 2 aa 100-200 (internal sequence).
    Database link: Q9H2A3
    (Peptide available as ab187850)

  • Positive control
    • WB: SH-SY5Y and HepG2 cell lysates. ICC/IF: SH-SY-5Y cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    Anti-Neurogenin 2 antibody [EPR4177] images

    • All lanes : Anti-Neurogenin 2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified)

      Lane 1 : PC-12 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 29 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-Neurogenin 2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 29 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Neurogenin 2 antibody [EPR4177] (ab109236) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa

    References for Anti-Neurogenin 2 antibody [EPR4177] (ab109236)

    ab109236 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample Mouse Tissue sections (Retina)
    Specification Retina
    Permeabilization Yes - 0.3% triton
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 20 2014

    Thank you for contacting Abcam.

    I am sorry that ab109236 is not producing a strong 25 kDa band in Western blot for your mouse brain homogenate. As we discussed on the phone, I am sending you a positive control free of charge. I also recommend ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"