Overview

  • Product nameAnti-Neurogenin3 antibody
    See all Neurogenin3 primary antibodies
  • Description
    Rabbit polyclonal to Neurogenin3
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Neurogenin3 aa 40-69 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • WB: Mouse liver tissue lysate. IHC-P: Human jejunum and large intestine tissues. ICC/IF: NCI-H460 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab38548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Block with 5% BSA.

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionInvolved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types.
  • Involvement in diseaseDefects in NEUROG3 are the cause of diarrhea type 4 (DIAR4) [MIM:610370]. DIAR4 is a characterized by severe, life-threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATH4B antibody
    • Atoh5 antibody
    • bHLHa7 antibody
    • Class A basic helix-loop-helix protein 7 antibody
    • Math4B antibody
    • NEUROG3 antibody
    • Neurogenin-3 antibody
    • NGN-3 antibody
    • ngn3 antibody
    • NGN3_HUMAN antibody
    • OTTHUMP00000019730 antibody
    • Protein atonal homolog 5 antibody
    see all

Anti-Neurogenin3 antibody images

  • Anti-Neurogenin3 antibody (ab38548) at 1/1000 dilution + Mouse liver tissue lysate

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa

    Incubation time was overnight at 4°C.

  • ab38548 (4µg/ml) staining Neurogenin-3 in human jejunum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human large intestine tissue labelling Neurogenin3 with ab38548. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • ab38548 staining Neurogenin3 in mouse psotnatal day 8 testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4$ paraformaldehyde and blocked with 5% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in 0.1M citric acid/sodium citrate. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A FITC-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of NCI-H460 cells labelling Neurogenin3 (green) with ab38548. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstained with DAPI (blue)

References for Anti-Neurogenin3 antibody (ab38548)

This product has been referenced in:
  • Pinney SE  et al. Neonatal diabetes and congenital malabsorptive diarrhea attributable to a novel mutation in the human neurogenin-3 gene coding sequence. J Clin Endocrinol Metab 96:1960-5 (2011). WB ; Human . Read more (PubMed: 21490072) »
  • Wilding Crawford L  et al. Gene expression profiling of a mouse model of pancreatic islet dysmorphogenesis. PLoS ONE 3:e1611 (2008). WB ; Mouse . Read more (PubMed: 18297134) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (1-2 month old testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acide/sodium citrate
Permeabilization No
Specification 1-2 month old testis
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative 4%PFA
Username

Abcam user community

Verified customer

Submitted Jan 14 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/sodium citrate
Sample Mouse Tissue sections (postnatal day 8 testes)
Specification postnatal day 8 testes
Permeabilization No
Fixative 4%PFA
Username

Dr. Qing Wen

Verified customer

Submitted May 08 2014

Thank you for contacting us and your interest in our products.

As you have observed from the datasheet of the Anti-Neurogenin3 antibody (ab38548) the immunogen used to raise the antibody is a 10-30 residue synthetic peptide taken from within ...

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Thank you for contacting us.

We have incubated the sections with primary antibody for 20 minutes at room temperature. However we recommend the users should incubate the antibody for at least 4 hours or overnight in case the staining is faint...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"