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Synthetic peptide corresponding to Human Neurogenin3 aa 40-69 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab38548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|WB||1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Block with 5% BSA.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Incubation time was overnight at 4°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human large intestine tissue labelling Neurogenin3 with ab38548. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
ab38548 staining Neurogenin3 in mouse psotnatal day 8 testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4$ paraformaldehyde and blocked with 5% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in 0.1M citric acid/sodium citrate. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A FITC-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence analysis of NCI-H460 cells labelling Neurogenin3 (green) with ab38548. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstained with DAPI (blue)