Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Mouse Neuroglycan C.
(Peptide available as ab32755.)
Our Abpromise guarantee covers the use of ab31946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | ||
IHC-FoFr | ||
WB |
ab31946 staining Neuroglycan C in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet.
ab31946 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"