Overview

  • Product name
  • Description
    Rabbit polyclonal to Neurogranin
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, IHC-Fr, WB, ICC, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Recombinant full length protein (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab23570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21346800
IHC-Fr 1/500. Fix fresh frozen sections with PFA.
WB 1/1000 - 1/2000. Predicted molecular weight: 7 kDa.
ICC 1/500.
IP 1/100.
ELISA 1/2000.

Target

  • Function
    Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
  • Tissue specificity
    In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
  • Sequence similarities
    Belongs to the neurogranin family.
    Contains 1 collagen-like domain.
    Contains 1 IQ domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein kinase C substrate RC3 antibody
    • Calmodulin binding protein antibody
    • Hng antibody
    • NEUG(55-78) antibody
    • NEUG_HUMAN antibody
    • Neurogranin (protein kinase C substrate) antibody
    • Ng antibody
    • NRGN antibody
    • Protein kinase C substrate antibody
    • RC3 antibody
    see all

Anti-Neurogranin antibody images

  • ab23570 at 1/500 staining mouse embryonic mesencephalon tissue sections by IHC-Fr. The sections were paraformaldehyde fixed and incubated with the antibody for 12 hours. An Alexa Fluor ®  488 conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

References for Anti-Neurogranin antibody (ab23570)

This product has been referenced in:

See all 3 Publications for this product

Product Wall

The recombinant protein immunogen for ab23570 did not contain any tag.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (cortical neurons)
Loading amount
30 µg
Specification
cortical neurons
Gel Running Conditions
Reduced Denaturing (4-12% SDS)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryonic mesencephalon)
Specification
embryonic mesencephalon
Fixative
Paraformaldehyde
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Aug 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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