Overview

  • Product nameAnti-Neurogranin antibody
    See all Neurogranin primary antibodies
  • Description
    Goat polyclonal to Neurogranin
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-KIKSGERGRKG

    , corresponding to internal sequence amino acids 45-55 of Human Neurogranin (NP_006167.1).

  • Positive control
    • Human brain cerebral cortex lysate, Human brain hippocampus lysate, Mouse brain lysate and Rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab99269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa). An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies.

Target

  • FunctionActs as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
  • Tissue specificityIn the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
  • Sequence similaritiesBelongs to the neurogranin family.
    Contains 1 collagen-like domain.
    Contains 1 IQ domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein kinase C substrate RC3 antibody
    • Calmodulin binding protein antibody
    • Hng antibody
    • NEUG(55-78) antibody
    • NEUG_HUMAN antibody
    • Neurogranin (protein kinase C substrate) antibody
    • Ng antibody
    • NRGN antibody
    • Protein kinase C substrate antibody
    • RC3 antibody
    see all

Anti-Neurogranin antibody images

  • All lanes : Anti-Neurogranin antibody (ab99269) at 2 µg/ml

    Lane 1 : Human cerebellum lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 8 kDa
  • Anti-Neurogranin antibody (ab99269) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 8 kDa
    Primary incubation was 1 hour.
  • ab99269 (3.8µg/ml) staining of paraffin embedded Human Cortex shows cytoplasm staining in the neuronal cell bodies. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-Neurogranin antibody (ab99269)

ab99269 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"