Recombinant
RabMAb

Anti-Neurokinin 1 Receptor antibody [EPR6836(2)] (ab183713)

Overview

  • Product name
    Anti-Neurokinin 1 Receptor antibody [EPR6836(2)]
    See all Neurokinin 1 Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR6836(2)] to Neurokinin 1 Receptor
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Neurokinin 1 Receptor aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P25103

  • Positive control
    • K562, Jurkat and THP-1 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR6836(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50 - 1/70.
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).

Target

  • Function
    This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Neurokinin 1 antibody
    • NK 1 receptor antibody
    • NK 1R antibody
    • NK-1 receptor antibody
    • NK-1R antibody
    • NK1 receptor antibody
    • NK1R antibody
    • NK1R_HUMAN antibody
    • NKIR antibody
    • SPR antibody
    • Substance P receptor antibody
    • Substance-P receptor antibody
    • TAC 1R antibody
    • TAC1R antibody
    • Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor) antibody
    • Tachykinin receptor 1 (substance P receptor neurokinin 1 receptor) antibody
    • Tachykinin receptor 1 antibody
    • TACR1 antibody
    see all

Images

  • Western blot analysis of Neurokinin 1 Receptor in K562 cell lysate immunoprecipitated using ab183713 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of K562 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

  • All lanes : Anti-Neurokinin 1 Receptor antibody [EPR6836(2)] (ab183713) at 1/50000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : THP-1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 46 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References

ab183713 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183713.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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