Anti-Neurokinin 3 Receptor NK3 antibody (ab124025)

Overview

  • Product nameAnti-Neurokinin 3 Receptor NK3 antibody
    See all Neurokinin 3 Receptor NK3 primary antibodies
  • Description
    Rabbit polyclonal to Neurokinin 3 Receptor NK3
  • Specificityab124025 does not recognise NK1R or NK2R.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    An 18 amino acid peptide from near the center of Human Neurokinin 3 Receptor NK3.

  • Positive control
    • Human brain tissue; RAW 264.7 whole cell lysate
  • General notesThis product should be stored undiluted.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab124025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 52 kDa).Can be blocked with Neurokinin 3 Receptor NK3 peptide (ab176411).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionThis is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K > substance K > substance P.
  • Involvement in diseaseHypogonadotropic hypogonadism 11 with or without anosmia
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    The anchoring of this receptor to the plasma membrane is probably mediated by the palmitoylation of a cysteine residue.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC148060 antibody
    • MGC148061 antibody
    • Neurokinin B receptor antibody
    • Neurokinin beta receptor antibody
    • Neuromedin K Receptor antibody
    • Neuromedin-K receptor antibody
    • NK 3 receptor antibody
    • NK 3R antibody
    • NK-3 receptor antibody
    • NK-3R antibody
    • NK3 receptor antibody
    • NK3R antibody
    • NK3R_HUMAN antibody
    • NKR antibody
    • TAC 3R antibody
    • TAC3R antibody
    • TAC3RL antibody
    • Tachykinin receptor 3 antibody
    • TACR 3 antibody
    • Tacr3 antibody
    see all

Anti-Neurokinin 3 Receptor NK3 antibody images

  • Lane 1 : Anti-Neurokinin 3 Receptor NK3 antibody (ab124025) at 0.5 µg/ml
    Lane 2 : Anti-Neurokinin 3 Receptor NK3 antibody (ab124025) at 2 µg/ml

    Lane 1 : RAW 264.7 whole cell lysate
    Lane 2 : RAW 264.7 whole cell lysate


    Predicted band size : 52 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • ab124025, at 5µg/ml, staining Neurokinin 3 Receptor NK3 in paraffin-embedded Human brain tissue by Immunohistochemistry.

References for Anti-Neurokinin 3 Receptor NK3 antibody (ab124025)

ab124025 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Human Tissue sections (Nasal mucosa)
Antigen retrieval step None
Permeabilization No
Specification Nasal mucosa
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
Fixative Zamboni
Username

Mr. Duc Dung Le

Verified customer

Submitted Apr 29 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Specification Brain
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Duc Dung Le

Verified customer

Submitted Apr 29 2015

Thank you for taking the time to complete our questionnaires and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from these antibodies.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"