Overview

  • Product name
    Anti-Neuroligin 2 antibody
    See all Neuroligin 2 primary antibodies
  • Description
    Goat polyclonal to Neuroligin 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide:

    NPPDTDIRDPGKK

    , corresponding to internal sequence amino acids 165-177 of Human Neuroligin 2 (NP_065846.1).

  • Positive control
    • Human Pancreas lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77595 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide.

Target

  • Relevance
    Neuroligins are cell adhesion proteins that are thought to instruct the formation and alignment of synaptic specializations. The three known rodent neuroligin isoforms share homologous extracellular acetylcholinesterase-like domains that bridge the synaptic cleft and bind beta-neurexins. All neuroligins have identical intracellular C-terminal motifs that bind to PDZ domains of various target proteins. Neuroligin 2 is exclusively localized to inhibitory synapses in rat brain and dissociated neurons. In immature neurons, neuroligin 2 is found at synapses and also at GABAA receptor aggregates that are not facing presynaptic termini.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • KIAA1366 antibody
    • MGC67386 antibody
    • NL2 antibody
    • NLGN2 antibody
    • Ortholog of human and rat neuroligin 2 NLGN2 antibody
    • OTTHUMP00000135277 antibody
    • OTTMUSP00000006214 antibody
    • RP23-422L16.24 antibody
    see all

Images

  • Anti-Neuroligin 2 antibody (ab77595) at 2 µg/ml + Human Pancrease lysate (in RIPA buffer) at 35 µg

    Predicted band size : 91 kDa

    Primary incubation was 1 hour.

    Detected by chemiluminescence.

References

ab77595 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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