Anti-Neuronal membrane glycoprotein M6-a antibody (ab109997)

Overview

  • Product nameAnti-Neuronal membrane glycoprotein M6-a antibody
    See all Neuronal membrane glycoprotein M6-a primary antibodies
  • Description
    Goat polyclonal to Neuronal membrane glycoprotein M6-a
  • SpecificityThis antibody is expected to recognize all reported isoforms (NP_005268.1; NP_963885.1; NP_963886.1). Reported variants represent identical protein: NP_963885.1, NP_005268.1.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide: C-

    QTYFEMARTAGD

    , corresponding to internal sequence amino acids 59-70 of Human neuronal membrane glycoprotein M6-a (NP_005268.1; NP_963885.1; NP_963886.1).

  • Positive control
    • Fetal mouse and Rat Brain lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 5 µg/ml. Predicted molecular weight: 31 kDa. An additional band of approx. 80kDa that corresponds to the glycosylated form was observed in Human, Mouse and Rat Brain lysates.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    Anti-Neuronal membrane glycoprotein M6-a antibody images

    • All lanes : Anti-Neuronal membrane glycoprotein M6-a antibody (ab109997) at 1 µg/ml

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : Human olfactory bulb tissue lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 31 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

      The observed 75kDa band corresponds to the glycosylated form.

    • All lanes : Anti-Neuronal membrane glycoprotein M6-a antibody (ab109997) at 2 µg/ml

      Lane 1 : Fetal mouse brain tissue lysate
      Lane 2 : Fetal rat brain tissue lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 31 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

      The observed 75kDa band corresponds to the glycosylated form.

    References for Anti-Neuronal membrane glycoprotein M6-a antibody (ab109997)

    ab109997 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"