Anti-Neuronal membrane glycoprotein M6-a antibody (ab79128)

Overview

  • Product name
    Anti-Neuronal membrane glycoprotein M6-a antibody
    See all Neuronal membrane glycoprotein M6-a primary antibodies
  • Description
    Rabbit polyclonal to Neuronal membrane glycoprotein M6-a
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Neuronal membrane glycoprotein M6-a.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Brain Membrane; Mouse Brain; Mouse Spinal Cord; Rat Brain; Rat Spinal Cord.

Properties

Applications

Our Abpromise guarantee covers the use of ab79128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

Images

  • All lanes : Anti-Neuronal membrane glycoprotein M6-a antibody (ab79128) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate
    Lane 6 : Spinal Cord (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab79128 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79128 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab79128 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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