The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 9 for isoform 1 , for isoform 2, 6 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay participate in the maintenance of segment identity in the hindbrain and pituitary development, and maturation or maintenance of the overall structure of the nervous system. May function as a regulatory subunit of ion channels.
Sequence similaritiesBelongs to the neuronatin family.
Developmental stageAbundant in 18-24 week old fetal brain. Postnatally its expression decline and only minimal levels were present in adulthood.