Overview

  • Product nameAnti-Neuropeptide S antibody
    See all Neuropeptide S primary antibodies
  • Description
    Rabbit polyclonal to Neuropeptide S
  • SpecificityThis antibody is generated against the complete 20aa sequence of the fully processed rat NPS peptide, it should run at about 2kDa on WB. ab18252 detects mouse NPS peptide in IHC (PFA fixed) and in RIA in hypothalamic mouse brain tissue.
  • Tested applicationsSuitable for: IHC (PFA fixed), RIA, IHC-FoFr, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of fully processed Rat Neuropeptide S.

    (Peptide available as ab18699.)

  • Positive control
    • Rat and Mouse brain

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use a concentration of 0.3 µg/ml.
RIA Use a concentration of 0.1 - 0.3 µg/ml.
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

Anti-Neuropeptide S antibody images

  • Immunofluorescent staining using the Neuropeptide S antibody (ab18252) in the rat hypothalamus. Picture taken using objectives X20 (A) or X40 (B). The staining is observed in thin and varicose fibers. This staining is blocked by preincubation with immunising peptides (ab18698 and ab18699). ab18252 was used at 1/1000 (0.3µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.

  • IC50: 46 fmoles; ab18252 used at a dilution of 1/3000; Tracer: [125I] Y10-hNPS, 12.000 cpm; Detection method: anti-rabbit SPA (Amersham).

    Radioimmunoassay was performed in equilibrium, using the SPA technology (Amersham). 50µl antirabbit YSi SPA beads (RPN 140) were mixed with 50µl antiserum (1:3000), 50µl tracer (12,000 cpm) and with or without cold NPS peptide in increasing concentrations. All reagents were resuspended in 0.1M phosphate buffer, pH 7, 0.1% BSA. Incubation was for 48h at RT. Bound radioligand was detected by scintillation proximity in a beta counter (MicroBeta, Wallac).

  • ab18252 staining Neuropeptide S in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 5% serum for 1 hour at 220C was performed. The sample was incubated with primary antibody (1/1000) for 10 hours at 220C. An Alexa Fluor® 555-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution. The images were generated from same slice and same area (KF, a part of LPB on mouse brain atlas). Scale bar is 200 µm on the left image and 20 µm on the right image. Blue colour represents DAPI staining, while red one is for Neuropeptide S.

    See Abreview

References for Anti-Neuropeptide S antibody (ab18252)

This product has been referenced in:
  • Liu X  et al. Molecular fingerprint of neuropeptide S-producing neurons in the mouse brain. J Comp Neurol 519:1847-66 (2011). Mouse . Read more (PubMed: 21452230) »
  • Clark SD  et al. Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry. J Comp Neurol 519:1867-93 (2011). Mouse . Read more (PubMed: 21452235) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3 Triton X-100, though not necessary
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Xiaobin Liu

Verified customer

Submitted Jul 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (rat brain)
Specification rat brain
Fixative Paraformaldehyde
Antigen retrieval step None
Username

Dr. Sophie Pezet

Verified customer

Submitted Dec 10 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"