Overview

  • Product name
    Neuropeptide S (rat)
  • Description
    NPS receptor agonist
  • Biological description
    Endogenous ligand for the NPS receptor involved in many biological functions including sleeping/wakening, locomotion, anxiety, and food intake.

Properties

Applications

Our Abpromise guarantee covers the use of ab120246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab70280 staining cAMP in HEK293 cells treated with neuropeptide S (ab120246), by ICC/IF. Increase in cAMP expression correlates with increased concentration of neuropeptide S, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120246 (neuropeptide S) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70280 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Reinscheid RK Neuropeptide S: anatomy, pharmacology, genetics and physiological functions. Results Probl Cell Differ 46:145-58 (2008). Read more (PubMed: 18204825) »
  • Xu YL  et al. Distribution of neuropeptide S receptor mRNA and neurochemical characteristics of neuropeptide S-expressing neurons in the rat brain. J Comp Neurol 500:84-102 (2007). Read more (PubMed: 17099900) »
  • Okamura N & Reinscheid RK Neuropeptide S: a novel modulator of stress and arousal. Stress 10:221-6 (2007). Read more (PubMed: 17613937) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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