Overview

  • Product nameAnti-Neuropeptide Y antibody [8]
    See all Neuropeptide Y primary antibodies
  • Description
    Mouse monoclonal [8] to Neuropeptide Y
  • SpecificityCross-reactivity with Neuropeptide Y(1-24), Neuropeptide Y (18-36), Peptide YY or Pancreatic Polypeptide was <0.2%.
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human/Rat Neuropeptide Y adsorbed onto aluminum hydroxide gel.

  • EpitopeThe epitope of ab112473 is probably around the mid-molecular region, amino acids 18-24 of Neuropeptide Y.
  • Positive control
    • Mouse brain tissue (fibres in the hilus of the hippocampus, following epiletic attack) Mouse brain tissue (interneurons in the cortex)

Properties

Applications

Our Abpromise guarantee covers the use of ab112473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/1000.

Target

Anti-Neuropeptide Y antibody [8] images

  • ab112473, at 1/1000, staining Neuropeptide Y in paraffin embedded Mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epiletic attack just before perfusion by Immunohistochemistry.
  • ab112473, at 1/1000, staining Neuropeptide Y in paraffin embedded Mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epiletic attack just before perfusion by Immunohistochemistry.
  • ab112473, at 1/1000, staining Neuropeptide Y in paraffin embedded Mouse brain tissue (interneurons in the cortex) by Immunohistochemistry.
  • Immunofluorescence images of  tissue from human cerebral cortex, applying ab112473 to ultra thin sections (antibody: green,

    DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil.

References for Anti-Neuropeptide Y antibody [8] (ab112473)

This product has been referenced in:
  • Xu X  et al. Altered expression of neuropeptide Y, Y1 and Y2 receptors, but not Y5 receptor, within hippocampus and temporal lobe cortex of tremor rats. Neuropeptides 48:97-105 (2014). IHC-P ; Mouse . Read more (PubMed: 24444822) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
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Submitted Sep 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"