Overview

  • Product nameAnti-Neuropeptide Y antibody
    See all Neuropeptide Y primary antibodies
  • Description
    Rabbit polyclonal to Neuropeptide Y
  • SpecificityThis antibody cross-reacts in dot blot with Peptide YY (PYY) conjugated to BSA. It shows no cross-reactivity in dot blot with Substance P, Neurokinin A, Neurokinin B, Vasoactive Intestinal Peptide (VIP), Calcitonin Gene-related Peptide (CGRP) (rat), Calcitonin, and Somatostatin conjugated to BSA, or BSA.
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IF, IHC-P, Dot blot, IHC-Fr, RIAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Monkey
  • Immunogen

    Full length Neuropeptide Y (porcine) conjugated to KLH.

  • Positive control
    • NPY-containing neurons (cell bodies, axons, and dendrites) in frozen sections of rat brain obtained following perfusion fixation with 4% paraformaldehyde. Formalin-fixed, frozen, or Vibratome sections of rat, cat, porcine, bovine, monkey, or human brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab10980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17455332Use at an assay dependent dilution. PMID 17455332.
ICC/IF Use at an assay dependent concentration. PubMed: 19609763
IHC-P 1/800.
Dot blot 1/10000. 1/10000. This concentration was determined using Neuropeptide Y-BSA (0.03-0.25 ng/dot).
IHC-Fr 1/8000. 1/8000. This concentration was determined using frozen sections of rat brain perfusion-fixed with 4% paraformaldehyde. Absorption studies show that specific staining of tissue may be inhibited following preincubation of the antiserum with 10 µM neuropeptide Y (porcine).
RIA 1/10000. 1/10000. This concentration was determined using 20 pg/tube of 125I-labeled neuropeptide Y in a second antibody and polyethylene glycol RIA. The sensitivity of the RIA assay is defined as the 90% intercept of a B/B0 standard curve. In this system the sensitivity has been found to be 20 pg/tube. The affinity constant (Ka) is determined by a Scatchard plot using this RIA system. Ka = Minimum 5.0 x 1010 L/mole

Target

Anti-Neuropeptide Y antibody images

  • ab10980 staining rat brain, striatum tissue sections by IHC-P.  Sections were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% serum for 2 hours at 20°C.  The primary antibody was diluted 1/800 and incubated with the sample for 14 hours at 20°C.  Arrows point to Neuropeptide Y positive interneurons. Magnification 20X.

    See Abreview

  • ab10980 staining Neuropeptide Y in chicken brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed with a Tris-EDTA buffer, pH 9. Samples were then permeabilized using 0.1% Triton X-100/PBS, blocked with 4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS for 1 hour at 4°C and then incubated with ab10980 at a 1/2000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

References for Anti-Neuropeptide Y antibody (ab10980)

This product has been referenced in:
  • Skärstrand H  et al. Neuropeptide Y is a minor autoantigen in newly diagnosed type 1 diabetes patients. Pediatr Diabetes N/A:N/A (2014). Human . Read more (PubMed: 25258030) »
  • Ohtsuka N  et al. GABAergic neurons regulate lateral ventricular development via transcription factor Pax5. Genesis 51:234-45 (2013). IHC-Fr . Read more (PubMed: 23349049) »

See all 9 Publications for this product

Product Wall

I am sorry for the delay in getting back to you, I now have further information to share with you in regards to the anti-Neuropeptide Y antibody (ab10980).

The antibody has been raised against the residues 10-45 of pig pro-neuropeptide. This ...

Read More

Thank you for contacting us.

I would like to share the reply I recieved from laboratory. "We have not tested productab10980 against the pro-peptide or in western blotting unfortunately. The antibody has been shown to cross-react with NPY ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue sections (Chicken brain cryosections)
Specification Chicken brain cryosections
Fixative Paraformaldehyde
Permeabilization Yes - PBS-0.1% Triton X-100
Blocking step 4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 02 2012

Thank you very much for your interest in our Neuropeptide Y antibodies.

As we have not tested any of these antibodies in western blot you are correct that they do not come under our Abpromsie, that does mean to say that they will not work, ju...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (pediatric brain)
Specification pediatric brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH8, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Dec 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain, striatum)
Specification brain, striatum
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% triton x-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3 - 1.0% Trtion X100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Michael Craig

Verified customer

Submitted Dec 11 2007

The immunogen used was full-length neuropeptide Y. Based on this, the antibody could recognize either the N-terminal or the C-terminal. Please contact us again if you have any additional questions.

Thank you for your enquiry. I have been in touch with the source of this antibody and I have determined that 1:8000 is the correct dilution for IHC-P using this antibody. The antibody was validated using IHC-P; detected using a HRP-streptavidin sys...

Read More

Thank you for your enquiry. At this time, we are unaware of any publications that feature the use of ab10980 (the references listed on the online datasheet are just general / background references). Ab10980 has been tested for application in IHC-fr...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"