The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/10000. (using the Biotin-Streptavidin/HRP detection method). Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~ 20 minutes. Note: If needed, low levels of glutaraldehyde (0.1 - 0.3%) may be used in conjunction with paraformaldehyde.
FunctionNPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Tissue specificityOne of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.