Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Pig Neuropeptide Y.
(Peptide available as ab32971.)
Our Abpromise guarantee covers the use of ab30914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunohistochemistical detection of Neuropeptide Y using ab30914 on formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)
Immunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.
ab30914 staining Neuropeptide Y in rat brain tissue section by IHC-FoFr (PFA perfusion fixed frozen sections). Rats were perfused with 4% PFA; tissue was fixed overnight in the same fixative and were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody dilution 1/3000 (PBS + 0.3% Triton X100) at 20°C for 18 hours. The Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG dilution 1/1000 was used as secondary antibody. The image shows staining in the soma and processes of neurons with the expected distribution of Neuropeptide Y.