• Product nameAnti-Neuropeptide Y antibody
    See all Neuropeptide Y primary antibodies
  • Description
    Sheep polyclonal to Neuropeptide Y
  • SpecificityThe specificity of the NPY antiserum was determined by radioimmunoassay. Synthetic peptides, somatostatin-14, leu-enkephalin, met-enkephalin, oxytocin, Arg8-vasopressin, vasoactive intestinal peptide, angiotensin II demonstrated less than 0.005% cross- reactivity and substance P less than 0.02%. Peptide YY and bovine pancreatic polypeptide demonstrated approx. 36% and 0.05% cross- reactivity respectively. Human peptide YY share 65% homology amino acid sequence for human NPY. Synthetic NPY completely abolished radio labelled NPY binding to NPY antibody in RIA and antibody binding to tissue antigen.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 30-64 of Human Neuropeptide Y. The peptide was coupled to Bovine Thyroglobulin.

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH.
  • General notesThis antibody is excellent for immunohistochemistry. Good staining was achieved in rabbit brain and guinea pig gastrointestinal tract.



Our Abpromise guarantee covers the use of ab6173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
IHC-FoFr 1/500. PubMed: 19200078


Anti-Neuropeptide Y antibody images

  • ab6173 staining Neuropeptide Y in rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/10000) for 16 hours at 4°C. A Biotin-conjugated Donkey anti-sheep IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab6173 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6173 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Neuropeptide Y antibody (ab6173)

This product has been referenced in:
  • Filipovic N  et al. Expression of PTHrP and PTH/PTHrP receptor 1 in the superior cervical ganglia of rats. Neuropeptides 48:353-9 (2014). Read more (PubMed: 25278090) »
  • Furlan A  et al. The transcription factor Hmx1 and growth factor receptor activities control sympathetic neurons diversification. EMBO J 32:1613-25 (2013). Read more (PubMed: 23591430) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: r.t°C
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde

Dr. Fabio Canneva

Verified customer

Submitted Jul 10 2014

We cannot guarantee this antibody works on paraffin embedded tissues. It has not been tested for this.

This antibody has not been tested for staining rat brain, so its reactivity with rat NPY is unknown, although certainly possible. You may be able to estimate the likelihood of cross-reactivity with this species by comparing the sequence of the syntheti...

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The affinity constant for this antibody has not been determined. The concentration of antibody in the sample has not been determined and the material is supplied as a whole serum. This specific antibody has been used for the past 15 or so years for man...

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