Anti-Neuropilin 1 (phospho T916) antibody (ab71766)


  • Product nameAnti-Neuropilin 1 (phospho T916) antibody
    See all Neuropilin 1 primary antibodies
  • Description
    Rabbit polyclonal to Neuropilin 1 (phospho T916)
  • SpecificityThis antibody recognizes ~130 kDa of Neuropilin 1 protein at the phosphorylation site of Thr 916. It does not react with non-phospho Neuropilin 1.
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    A synthetic peptide surrounding the epitope LNTQS with a phosphorylation site at Thr916. This sequence is identical between human and cow.



Our Abpromise guarantee covers the use of ab71766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 103 kDa. The endogenous phosphspecific neuropilin-1 expression is weak, and IP can enrich the protein for detection in WB.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use at an assay dependent concentration.


  • FunctionThe membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificityThe expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similaritiesBelongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A5 protein antibody
    • BDCA4 antibody
    • CD304 antibody
    • Neuropilin-1 antibody
    • Neuropilin1 antibody
    • NP1 antibody
    • NPN1 antibody
    • NRP 1 antibody
    • NRP antibody
    • NRP1 antibody
    • NRP1_HUMAN antibody
    • transmembrane receptor antibody
    • Vascular endothelial cell growth factor 165 receptor antibody
    • VEGF165R antibody
    see all

Anti-Neuropilin 1 (phospho T916) antibody images

  • Predicted band size : 103 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • Neuropilin 1 (phospho T916) antibody (ab71766) at 1/1000 dilution recognizing Neuropilin 1 protein phosphorylated at Thr916 (Lane 1, A). Lane 2 shows a non-Abcam Neuropilin 1 antibody.
    1 µg of the following peptides was blotted onto NC membrane:
    A: Neuropilin 1 (Phosphospecific peptide at pT916)
    B: Neuropilin1 (Nonphospho PP)
    C: Non-related phosphospecific PP

References for Anti-Neuropilin 1 (phospho T916) antibody (ab71766)

ab71766 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply and for sending your protocol and image.

You are using the maximum recommended amount of the antibody. Try diluting the primary more to 1/5000 and the secondary to 1/10,000. Include 5% BSA in with the primary and seco...

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Thank you for your reply. I am sorry that this antibody is producing poor results in IP. I would be happy to review your results and protocol to see if there are any suggestions I can make to help troubleshoot. If you can please provide your data, I wi...

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Thank you for your inquiry and your patience with this reply as I was following up with the lab for more information.

It may be possible that the ab29466 could be used in an IP, but you raise a good point, and since it isn't tested in IP we w...

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I want to recommend a lysate of HUVEC, based on some blot images I have seen for total neuropilin. I think there will be some constituitive phosphorylation but I have not seen data confirming this, just evidence of neuropilin expression. We do not at t...

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Thank you for your reply.

We sell human brain tissue lysate as ab29466, but this is something you would have to purchase.

But we are running a...

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Thank you for your reply.

Since you purchased this antibody through our distributor, we will send the replacement vial of ab71766 to them on their next Abcam orderand they will forward it to you.

The lab finally got back ...

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Thank you for contacting Abcam regarding ab71766.

Below please find the recommended protocol for detecting phospho-specific proteins.

Sample preparation:

1. Homogenize the cells or tissue of interest in l...

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