All tags neuroscience Immature neuron and intermediate progenitor markers

Immature neuron and intermediate progenitor markers

A guide to choosing markers for immature neurons and intermediate progenitors

Radial glia divide asymmetrically to produce one radial glia cell and one intermediate progenitor cell (IPC). IPCs differentiate into post-mitotic immature neurons, which migrate to their final destination in the nervous system and integrate into the neuronal network.

Intermediate progenitors

TBR2

A transcription factor whose expression marks the transition from radial glia to intermediate progenitors.

Embryonic (E15) mouse cortical sections stained with anti-TBR2 (red) (ab23345).

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MASH1 (Ascl1)

A transcription factor essential for neural differentiation. Please note that it can also label active neural stem cells.

Mouse subventricular zone tissue sections stained with anti-MASH1 (ab74065).

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Immature neurons

Doublecortin

A microtubule-associated phosphoprotein that promotes neurite extension and cell migration.

Mouse adult dentate gyrus sections stained with anti-doublecortin (red) (ab18723).

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Beta III tubulin

A class of tubulin whose expression is limited to neurons and is used to label immature neurons.

Mouse embryonic spinal cord stained with anti-beta III tubulin (red) (ab78078).

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NeuroD1

A transcription factor that promotes neuronal development.

Mouse brain tissue stained with anti-neuroD1 (ab60704).

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TBR1

A transcription factor whose expression marks the transition from intermediate progenitors to post-mitotic neurons.

Mouse brain sections stained with anti-TBR1 (red) (ab31940).

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Stathmin 1

A cytoplasmic phosphoprotein involved in cytoskeletal regulation.

Mouse brain tissue sections stained with anti-stathmin 1 (ab109986).

Browse all stathmin 1 antibodies

References

  • Atweh, C. The Role of Stathmin in the Regulation of the Cell Cycle. J.Cell.Biochem 93, (2004).
  • Englund, C. et al. Pax6, Tbr2, and Tbr1 Are Expressed Sequentially by Radial Glia, Intermediate Progenitor Cells, and post-mitotic Neurons in Developing Neocortex. J. Neurosci. 25, 247–251 (2005).
  • Katsetos, C. D. et al. Class III beta-tubulin isotype (beta III) in the adrenal medulla: I. Localization in the developing human adrenal medulla. Anat. Rec. 250, 335–43 (1998).
  • Kim, E. J., Ables, J. L., Dickel, L. K., Eisch, A. J. & Johnson, J. E. Ascl1 (Mash1) Defines Cells with Long-Term Neurogenic Potential in Subgranular and Subventricular Zones in Adult Mouse Brain. (2011).
  • Kwak, M. et al. Effects of Neonatal Hypoxic-Ischemic Injury and Hypothermic Neuroprotection on Neural Progenitor Cells in the Mouse Hippocampus. Dev. Neurosci. 37, 428–439 (2015).
  • Martí-Mengual, U., Varea, E., Crespo, C., Blasco-Ibáñez, J. M. & Nacher, J. Cells expressing markers of immature neurons in the amygdala of adult humans. Eur. J. Neurosci. 37, 10–22 (2013).
  • Menezes, J. R. & Luskin, M. B. Expression of neuron-specific tubulin defines a novel population in the proliferative layers of the developing telencephalon. J. Neurosci. 14, 5399–5416 (1994).
  • Pollen, A. A. et al. Molecular Identity of Human Outer Radial Glia during Cortical Development. Cell 163, 55–67 (2015).
  • Shin, J. et al. Single-Cell RNA-Seq with Waterfall Reveals Molecular Cascades underlying Adult Neurogenesis. Cell Stem Cell 17, 360–372 (2015).
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