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Neuroepithelial cell markers

A guide to choosing markers to label neuroepithelial cells

Neuroepithelial (NE) cells are symmetrically dividing cells that form the neural plate and neural tube during embryonic development. They exhibit typical epithelial features such as tight junctions and are highly polarized along their apical-basal axis.  


Nestin
An intermediate filament protein expressed in NE cells. Its expression persists in radial glia until astrocyte development.

 iPSCs stained with anti-nestin (red)(ab105389).

Browse all nestin antibodies


SOX2
A transcription factor and the earliest marker of the neural plate. It is expressed in proliferating cells and those that acquire glial fates, but downregulated in post-mitotic neurons.

E13 mouse spinal cord sections stained with anti-SOX2 (ab97959).

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Notch1

A transmembrane receptor that regulates the formation, migration, and differentiation of neural crest cells.

Mouse embryo tissue sections stained with  anti-notch1 (green) (ab65297).

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Notch signaling inhibitor: FLI-06 (ab146170)


HES1 and HES3

Transcription factors that maintain the symmetrical cell division of NE cells. When NE cells become radial glia, HES3 is downregulated and HES5 is upregulated.

Mouse  NIH3T3 cells stained with anti-HES1 (green) (ab71559).

Browse all HES1 antibodies
Browse all HES3 antibodies


Occludin
A tight junction protein lost after neural tube closure prior to neurogenesis.

Human breast cancer cells stained with  anti-occludin (red) (ab31721).

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E-cadherin
Cell adhesion molecule downregulated after neural tube closure prior to neurogenesis.

Human  pluripotent stem cells stained with anti-E-cadherin (ab40772).

Browse all E-cadherin antibodies


SOX10

A transcription factor present in migrating neural crest cells.

A375 cells stained with anti-SOX10 (ab155279).

Browse all SOX10 antibodies

References

  • Bylund, M., Andersson, E., Novitch, B. G. & Muhr, J. Vertebrate neurogenesis is counteracted by Sox1-3 activity. Nat Neurosci 6, 1162–1168 (2003).
  • Jessen, K. R. & Mirsky, R. The origin and development of glial cells in peripheral nerves. Nat. Rev. Neurosci. 6, 671–82 (2005).
  • Krämer, A. et al. Small molecules intercept Notch signaling and the early secretory pathway. Nat. Chem. Biol. 9, 731–8 (2013).
  • Kageyama, R., Ohtsuka, T. & Kobayashi, T. Roles of Hes genes in neural development. Development Growth and Differentiation 50, (2008).
  • Kriegstein, A. R. & Götz, M. Radial glia diversity: A matter of cell fate. Glia 43, 37–43 (2003).
  • Noisa, P., Lund, C., Kanduri, K., Lund, R. & La, H. Notch signaling regulates the differentiation of neural crest from human pluripotent stem cells. J. Cell Sci. 127, 2083–2094 (2014).
  • Papanayotou, C. et al. A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. Plos Biol 6, e2 (2008).
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