The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.
Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Plasma membrane. Integral membrane protein.
Top image: Light microscopic analysis of NTSR1 expression in primary afferent neurons (arrows) using 1:1000 ab66216 of PFA fixed cells. Bottom image: At higher magnification, neurons exhibit a cytoplasmic pattern of immunoreactivity (arrowheads).
Western blot - Neurotensin antibody (ab66216)
All lanes : Anti-Neurotensin Receptor 1 antibody (ab66216) at 1/5000 dilution
Lane 1 : DRG's Lane 2 : Lumbar spinal chord Lane 3 : brain
Lysates/proteins at 25 µg per lane.
Predicted band size : 47 kDa Observed band size : 47 kDa