Anti-Neurotensin Receptor 1 antibody (ab75555)

Overview

  • Product name
    Anti-Neurotensin Receptor 1 antibody
    See all Neurotensin Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to Neurotensin Receptor 1
  • Specificity
    ab75555 detects endogenous levels of total Neurotensin receptor 1 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from an internal sequence of human Neurotensin Receptor 1

  • Positive control
    • Extracts from Jurkat cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
Indirect ELISA 1/5000.

Target

  • Relevance
    Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.
  • Cellular localization
    Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Plasma membrane. Integral membrane protein.
  • Database links
  • Alternative names
    • High affinity levocabastine insensitive neurotensin receptor antibody
    • Neurotensin receptor 1 (high affinity) antibody
    • Neurotensin receptor type 1 antibody
    • NT R 1 antibody
    • NTR antibody
    • NTR1 antibody
    • NTR1_HUMAN antibody
    • NTRH antibody
    • NTRR antibody
    • NTSR 1 antibody
    see all

Anti-Neurotensin Receptor 1 antibody images

  • All lanes : Anti-Neurotensin Receptor 1 antibody (ab75555) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ICC/IF image of ab75555 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Neurotensin Receptor 1 antibody (ab75555)

ab75555 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
15 µg
Specification
brain
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up