Anti-Neurotensin Receptor 1 antibody (ab75555)

Overview

  • Product nameAnti-Neurotensin Receptor 1 antibody
    See all Neurotensin Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to Neurotensin Receptor 1
  • Specificityab75555 detects endogenous levels of total Neurotensin receptor 1 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from an internal sequence of human Neurotensin Receptor 1

  • Positive control
    • Extracts from Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
Indirect ELISA 1/5000.

Target

  • RelevanceNeurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.
  • Cellular localizationCell Membrane, Endoplasmic reticulum, Golgi Apparatus and Plasma membrane. Integral membrane protein.
  • Database links
  • Alternative names
    • High affinity levocabastine insensitive neurotensin receptor antibody
    • Neurotensin receptor 1 (high affinity) antibody
    • Neurotensin receptor type 1 antibody
    • NT R 1 antibody
    • NTR antibody
    • NTR1 antibody
    • NTR1_HUMAN antibody
    • NTRH antibody
    • NTRR antibody
    • NTSR 1 antibody
    see all

Anti-Neurotensin Receptor 1 antibody images

  • All lanes : Anti-Neurotensin Receptor 1 antibody (ab75555) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ICC/IF image of ab75555 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Neurotensin Receptor 1 antibody (ab75555)

ab75555 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 15 µg
Specification brain
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"