Anti-Neurotrophin 3 antibody (ab53685)

Overview

  • Product name
    Anti-Neurotrophin 3 antibody
    See all Neurotrophin 3 primary antibodies
  • Description
    Rabbit polyclonal to Neurotrophin 3
  • Specificity
    ab53685 shows reactivity of less than 0.1% with mouse NGF, recombinant human BDNF or Neurotrophin 4.
  • Tested applications
    Suitable for: WB, ELISA, Inhibition Assay, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 139-149 of Mouse Neurotrophin 3 conjugated to BSA.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 30 kDa.
ELISA Use a concentration of 1 - 10 µg/ml.
Inhibition Assay Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.

Target

Anti-Neurotrophin 3 antibody images

  • All lanes : Anti-Neurotrophin 3 antibody (ab53685) at 1/500 dilution

    Lane 1 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 2 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 3 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 4 : Rat Brain (Age P21) Whole Tissue ysate

    Secondary
    Dako Cytomation P0260 Conjugation: HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

References for Anti-Neurotrophin 3 antibody (ab53685)

ab53685 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (day 11,5 Embryo)
Permeabilization
No
Specification
day 11,5 Embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Mr. Pierre-Yves Canto

Verified customer

Submitted Aug 29 2016

Thank you for your inquiry. This antibody to NT3 has been tested for biological activity using an in vitro assay based on the culture of embryonic sensory neurons from the chick dorsal root ganglia. The assay is performed according to the reference...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain Whole Tissue Lysate)
Loading amount
10 µg
Specification
Brain Whole Tissue Lysate
Gel Running Conditions
Reduced Denaturing (15% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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