Overview

  • Product nameNeurotrophin 3 Human ELISA kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.25 87% - 105%
    Serum 96.98 85% - 103%
    Plasma 94.45 84% - 105%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Neurotrophin 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 3 in serum, plasma, and cell culture supernatants. (Human Neurotrophin 3 concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for Human Neurotrophin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceNeurotrophin 3 (NT3) is part of the family of neurotrophic factors that control survival and differentiation of mammalian neurons. NT3 is closely related to both nerve growth factor and brain derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and affect development of neurons in the embryo when it is expressed in human placenta. NT3 deficient mice generated by gene targeting display severe movement defects of the limbs.
  • Alternative names
    • AI316846
    • AI835689
    • HDNF
    • MGC129711
    • Nerve growth factor 2
    • Neurotrophic factor
    • Neurotrophin 3 (HDNF/NT 3)
    • Neurotrophin 3 precursor
    • Neurotrophin3
    • NGF 2
    • NGF2
    • NT3
    • NTF 3
    • NTF3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Neurotrophin 3 Human ELISA kit images

  • NT-3 measured in biologicals showing quantity (pg) per mL tested sample

  • Representative Standard Curve using ab100615.

  • Representative Standard Curve using ab100615.

Protocols

References for Neurotrophin 3 Human ELISA kit (ab100615)

ab100615 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. Please find attached the Certificate of Compliance for ab100615 as requested. If there is anything else I can help you with, please let me kn

Thank you for your inquiry. We haven't tested ab100615 with cell lysates.  It would most likely work, but we wouldn't be able to guarantee it since it is untested.   If you decide to test this kit with lysate samples, we recommend diluting the samp...

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Thank you very much for your interest in ab100615. This kit has not been validated for use with mouse samples, but is predicted to react based on high sequence homology. I can offer a discount off a future purchase if you buy either this ELI...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"