Anti-Neurotrypsin antibody - Catalytic domain (ab59454)


  • Product name
    Anti-Neurotrypsin antibody - Catalytic domain
  • Description
    Rabbit polyclonal to Neurotrypsin - Catalytic domain
  • Specificity
    Recognizes the 875 and 834 amino acid forms of neurotrypsin. We have a range of domain specific antibodies for this target. For a full list please see all Neurotrypsin antibodies.
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide based on the catalytic domain of human Neurotrypsin.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab59454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 97 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.


  • Function
    Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations.
  • Tissue specificity
    Brain and Leydig cells of the testis.
  • Involvement in disease
    Defects in PRSS12 are the cause of mental retardation autosomal recessive type 1 (MRT1) [MIM:249500]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
    Contains 4 SRCR domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BSSP 3 antibody
    • BSSP3 antibody
    • Leydin antibody
    • MGC12722 antibody
    • Motopsin antibody
    • MRT1 antibody
    • NETR_HUMAN antibody
    • Neurotrypsin antibody
    • protease, serine, 12 (neurotrypsin, motopsin) antibody
    • Prss12 antibody
    • Serine protease 12 antibody
    see all

Anti-Neurotrypsin antibody - Catalytic domain images

  • All lanes : Anti-Neurotrypsin antibody - Catalytic domain (ab59454) at 1/1000 dilution

    Lane 1 : Tissue lysate prepared from rat testis at 15 µl
    Lane 2 : Tissue lysate prepared from rat testis at 10 µl
    Lane 3 : Tissue lysate prepared from mouse testis at 10 µl
    Lane 4 : Tissue lysate prepared from mouse testis at 8 µl
    Lane 5 : Tissue lysate prepared from mouse testis at 6 µl

    Predicted band size : 97 kDa

References for Anti-Neurotrypsin antibody - Catalytic domain (ab59454)

This product has been referenced in:
  • Meliopoulos VA  et al. MicroRNA regulation of human protease genes essential for influenza virus replication. PLoS One 7:e37169 (2012). WB ; Human . Read more (PubMed: 22606348) »

See 1 Publication for this product

Product Wall

Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
Mouse Tissue sections (brain hemisphere coronal sections)
brain hemisphere coronal sections
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2 · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 15 2013

Thank you very much for your interest our AbTrial program.

To our knowledge, ab59454 has not been tested in IHC-p or IHC-Fr. However by participating in our AbTrial program you can now use our products in an untested application or species w...

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According to our records, ab50454 was proving difficult to use inWestern blotand we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand th...

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Thank you for contacting us and letting us know about the trouble with this antibody. I do have a couple of additional questions so that I can better understand the situation. Did you also probe for a loading control like beta actin? What is the differ...

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