Overview

  • Product name
  • Description
    Rabbit polyclonal to Neurturin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 178-193 of Human Neurturin (Peptide available as ab8393.)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 14 kDa.Can be blocked with Neurturin peptide (ab8393).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
  • Involvement in disease
    Defects in NRTN are a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
  • Sequence similarities
    Belongs to the TGF-beta family. GDNF subfamily.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Neurturin antibody
    • Neurturin precursor antibody
    • NRTN antibody
    • NRTN_HUMAN antibody
    • NTN antibody
    • prepro-neurturin antibody
    see all

Images

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling Neurturin with Anti-Neurturin antibody (ab8061) at 5μg/ml.
  • All lanes : Anti-Neurturin antibody (ab8061) at 1/500 dilution

    Lane 1 : 10ng full length recombinant Neurturin in HeLa cell lysate
    Lane 2 : 2.5ng full length recombinant Neurturin in HeLa cell lysate
  • ab8061 at 5µg/ml staining Neurturin in human brain tissue by IHC.

References

This product has been referenced in:
  • Samiotaki G  et al. Enhanced delivery and bioactivity of the neurturin neurotrophic factor through focused ultrasound-mediated blood--brain barrier opening in vivo. J Cereb Blood Flow Metab 35:611-22 (2015). Read more (PubMed: 25586140) »
  • Wang K  et al. The neurotrophic factor neurturin contributes toward an aggressive cancer cell phenotype, neuropathic pain and neuronal plasticity in pancreatic cancer. Carcinogenesis N/A:N/A (2013). IHC ; Human . Read more (PubMed: 24067900) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Recombinant protein (Extracts of transfected hNRTN COS-1 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
Extracts of transfected hNRTN COS-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

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Verified customer

Submitted Aug 07 2015

Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer xx und so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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