Overview

  • Product name
    Anti-Neutrophil antibody [7/4]
    See all Neutrophil primary antibodies
  • Description
    Rat monoclonal [7/4] to Neutrophil
  • Specificity
    Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Neutrophil.

  • Epitope
    Recognizes the Ly-6B.2 antigen.
  • Positive control
    • Flow cytometry: Mouse peripheral blood granulocytes.
  • General notes

    Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53457 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    7/4
  • Myeloma
    Y3/Ag1.2.3
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 25505239
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Cellular localization
    Cell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Images

  • Staining of mouse peripheral blood granulocytes with Rat anti mouse neutrophil (ab53457).

References

This product has been referenced in:
  • Amrouche L  et al. MicroRNA-146a in Human and Experimental Ischemic AKI: CXCL8-Dependent Mechanism of Action. J Am Soc Nephrol 28:479-493 (2017). Read more (PubMed: 27444565) »
  • Cheng Z  et al. Anti-Inflammatory Mechanism of Neural Stem Cell Transplantation in Spinal Cord Injury. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27563878) »

See all 9 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 12 2016

Thank you for contacting us this afternoon and reporting the problems encountered with the most recent lot of the Anti-Neutrophil antibody [7/4] (ab53457). I am sorry to hear this antibody is not providing satisfactory results.

As discussed ...

Read More

Thank you for contacting us.

This product has not been tested in that particular strain. However it is covered by our Abpromise and guaranteed to work on mouse in any of the tested applications listed on the datasheet.

I hope this...

Read More

Thank you for contacting us.

Thank you for your inquiry. We have not tested MCA771GA on the Vm/Dk mouse strain. We ran a literature search, butwere unable to find any publications in which customers have tried this antibody on this mouse str...

Read More

Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying t...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up