Anti-Neutrophil antibody [7/4] (ab53457)
Key features and details
- Rat monoclonal [7/4] to Neutrophil
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-Neutrophil antibody [7/4]
See all Neutrophil primary antibodies -
Description
Rat monoclonal [7/4] to Neutrophil -
Host species
Rat -
Specificity
Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity. -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Neutrophil. Cultured bone marrow cells
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Epitope
Recognizes the Ly-6B.2 antigen. -
Positive control
- Flow cytometry: Mouse peripheral blood granulocytes.
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General notes
Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.
ab53457 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7/4 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 25505239
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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IHC-P
Use at an assay dependent concentration. PubMed: 25505239 |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Database links
- Entrez Gene: 110453 Mouse
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Alternative names
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
Datasheets and documents
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Datasheet download
References (34)
ab53457 has been referenced in 34 publications.
- McClellan BL et al. Murine brain tumor microenvironment immunophenotyping using mass cytometry. STAR Protoc 3:101357 (2022). PubMed: 35634359
- Earnest JT et al. The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell Rep 35:108962 (2021). PubMed: 33826892
- Li Y et al. Nuclear envelope rupture and NET formation is driven by PKCa-mediated lamin B disassembly. EMBO Rep 21:e48779 (2020). PubMed: 32537912
- Zaidan M et al. Signaling pathways predisposing to chronic kidney disease progression. JCI Insight 5:N/A (2020). PubMed: 32376805
- Ji W et al. Majie cataplasm provides a shield against asthmatic punch from the neuroimmune system. Heliyon 6:e03896 (2020). PubMed: 32478182