Anti-Neutrophil antibody [7/4] (FITC) (ab53453)

Overview

  • Product nameAnti-Neutrophil antibody [7/4] (FITC)
    See all Neutrophil primary antibodies
  • Description
    Rat monoclonal [7/4] to Neutrophil (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityRat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: cultured bone marrow cells.

  • EpitopeRecognizes the Ly-6B.2 antigen.
  • Positive control
    • Flow Cyt.: New Zealand Black mouse peripheral blood granulocytes.
  • General notes

    ab53453 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53453 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody notesab53453 recognises a neutrophil allotypic marker, a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages.
  • ClonalityMonoclonal
  • Clone number7/4
  • MyelomaY3/Ag1.2.3
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20038794
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Use 10µl of the working dilution to label 106 cells in 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [7/4] (FITC) images

  • Immunohistochemical analysis of acetone-fixed frozen mouse spleen, labelling Neutrophil with ab53453 at a dilution of 1/100 incubated for 1 hour at 25°C in 5% goat serum in TBS. The secondary used (to amplify the signal) was a Goat anti-rat polyclonal Alexa Fluor® 488 conjugate at a 1/400 dilution.

    See Abreview

  • Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti mouse neutrophil:FITC (ab53453).

References for Anti-Neutrophil antibody [7/4] (FITC) (ab53453)

This product has been referenced in:
  • Lamagna C  et al. B cell-specific loss of Lyn kinase leads to autoimmunity. J Immunol 192:919-28 (2014). Read more (PubMed: 24376269) »
  • Claudepierre MC  et al. Yeast virus-derived stimulator of the innate immune system augments the efficacy of virus vector-based immunotherapy. J Virol 88:5242-55 (2014). Flow Cyt . Read more (PubMed: 24574403) »

See all 9 Publications for this product

Product Wall

Swiss PO is used to denote "Swiss-Pathology Oxford," a specific strain at Oxford University.

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive neutrophils
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Verified customer

Submitted Nov 12 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive granulocytes
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Verified customer

Submitted Apr 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive monocytes
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Submitted Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen from immune mouse)
Permeabilization No
Specification Spleen from immune mouse
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Acetone
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Submitted Apr 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from blood)
Specification cells from blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy neutrophils
Username

Abcam user community

Verified customer

Submitted Dec 20 2011

Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope ...

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"