PE Anti-Neutrophil antibody [7/4] (ab105157)
Key features and details
- PE Rat monoclonal [7/4] to Neutrophil
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE Anti-Neutrophil antibody [7/4]
See all Neutrophil primary antibodies -
Description
PE Rat monoclonal [7/4] to Neutrophil -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Neutrophil. Cultured mouse bone marrow cells.
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Epitope
Recognizes the Ly-6B.2 antigen. -
General notes
ab105157 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.
ab105157 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7/4 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 10µl for 106 cells. ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Database links
- Entrez Gene: 110453 Mouse
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Alternative names
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab105157 has been referenced in 5 publications.
- Cai Z et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). PubMed: 27444869
- Rosas M et al. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol 88:169-80 (2010). PubMed: 20400676
- Locke LW et al. A novel neutrophil-specific PET imaging agent: cFLFLFK-PEG-64Cu. J Nucl Med 50:790-7 (2009). PubMed: 19372473
- Word RA et al. Transgene insertion on mouse chromosome 6 impairs function of the uterine cervix and causes failure of parturition. Biol Reprod 73:1046-56 (2005). PubMed: 16034000
- Hirsch S & Gordon S Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4. Immunogenetics 18:229-39 (1983). PubMed: 6618532