Anti-Neutrophil antibody [7/4] (Phycoerythrin) (ab105157)


  • Product nameAnti-Neutrophil antibody [7/4] (Phycoerythrin)
    See all Neutrophil primary antibodies
  • Description
    Rat monoclonal [7/4] to Neutrophil (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityRat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Cultured Mouse bone marrow cells.

  • EpitopeRecognizes the Ly-6B.2 antigen.
  • General notes

    ab105157 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab105157 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.



  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone number7/4
  • MyelomaY3/Ag1.2.3
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab105157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.


  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [7/4] (Phycoerythrin) images

  • Mouse (strain C57BL6) peripheral granulocytes stained with ab105157.

References for Anti-Neutrophil antibody [7/4] (Phycoerythrin) (ab105157)

This product has been referenced in:
  • Cai Z  et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). Read more (PubMed: 27444869) »
  • Rosas M  et al. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol 88:169-80 (2010). Read more (PubMed: 20400676) »

See all 5 Publications for this product

Product Wall

You contacted Abcam back in November 2011 about a problem with ab105157 in flow cytometry. I was wondering if the problems had been resolved or if you are still having problems with it.

Please let me know if there is anything I can help you w...

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Thank you for your inquiry. The concentration of specific antibody in this product cannot be accurately calculated because of the interference in the reading from the PE protein itself. We can estimate that this product is 0.1mg/ml, but again, this...

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Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope ...

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Thank you for contacting us and for including your attached flow data. I agree the vial of ab105157 you received is not working as expected. If you could forward me the purchase order number or Abcam order reference number associated with the purchase ...

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Thank you for contacting Abcam. I am sorry that the current lot of ab105157 is not working in flow cytometry. The data you provide clearly shows that there is a difference in the results between the old and the new antibody. Would you be able to send m...

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying t...

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