Anti-Neutrophil antibody [NIMP-R14] (Phycoerythrin) (ab125159)

Overview

  • Product nameAnti-Neutrophil antibody [NIMP-R14] (Phycoerythrin)
    See all Neutrophil primary antibodies
  • Description
    Rat monoclonal [NIMP-R14] to Neutrophil (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Functional Studies, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Purified BALB/c Mouse neutrophils.

  • EpitopeThe monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
  • Positive control
    • Mouse spleen tissue; Mouse neutrophils.
  • General notes

     

     

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberNIMP-R14
  • IsotypeIgG2b
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab125159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Blocking with 20% normal rabbit serum.
IHC-Fr Use at an assay dependent concentration. Tissue was fixed with acetone.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 10µl for 105 cells. ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23109724

Target

  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

References for Anti-Neutrophil antibody [NIMP-R14] (Phycoerythrin) (ab125159)

This product has been referenced in:
  • Menten-Dedoyart C  et al. Neutrophil extracellular traps entrap and kill Borrelia burgdorferi sensu stricto spirochetes and are not affected by Ixodes ricinus tick saliva. J Immunol 189:5393-401 (2012). ICC/IF ; Mouse . Read more (PubMed: 23109724) »
  • de Vries B  et al. Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 170:3883-9 (2003). Read more (PubMed: 12646657) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Permeabilization No
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"