Overview

  • Product name
  • Description
    Rabbit polyclonal to Nexilin
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide within internal sequence amino acids 252-301: EELERQRQEN RKKQAEEEAR KRLEEEKRAF EEARRQMVNE DEENQDTAKI, of Human Nexilin (NP_653174)

  • Positive control
    • Fetal liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83746 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.
  • Tissue specificity
    Abundantly expressed in heart and skeletal muscle, and at lower levels in placenta, lung, liver and pancreas. Also expressed in HeLa S3 and Molt-4 cells.
  • Involvement in disease
    Defects in NEXN are the cause of cardiomyopathy dilated type 1CC (CMD1CC) [MIM:613122]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in NEXN are the cause of cardiomyopathy familial hypertrophic type 20 (CMH20) [MIM:613876]. CMH20 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similarities
    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cytoplasm > myofibril > sarcomere > Z line. Localizes to the cell-matrix AJ. Not found at the cell-cell AJ.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110046H09Rik antibody
    • AA553326 antibody
    • F-actin binding protein antibody
    • F-actin-binding protein antibody
    • MGC104234 antibody
    • MGC138865 antibody
    • MGC138866 antibody
    • Nelin antibody
    • Nexilin antibody
    • Nexn antibody
    • NEXN_HUMAN antibody
    • nF actin binding protein antibody
    • OTTHUMP00000038336 antibody
    see all

Images

  • Anti-Nexilin antibody (ab83746) at 1 µg/ml (in 5% skim milk/ PBS) + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 39 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab83746 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83746, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbir IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Soni S  et al. A Proteomics Approach to Identify New Putative Cardiac Intercalated Disk Proteins. PLoS One 11:e0152231 (2016). IHC . Read more (PubMed: 27148881) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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