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Synthetic peptide corresponding to Human NF-kB p65 (internal sequence) (acetyl K310).
Our Abpromise guarantee covers the use of ab52175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
ICC/IF image of ab52175 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52175, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence analysis of ab52175 stained HeLa cells.The picture on the right is treated with the synthesized peptide.
Image courtesy of Tom Hu by Abreview.
ab52175 used at a 1/1000 dilution in Western Blot.Whole tissue lysate prepared from murine sciatic nerves was loaded at 200µg.Performed under reduced, denaturing conditions.Blocking step 5% milk for 1 hour at 20°C.Secondary used was an HRP conjugated goat anti-rabbit IgG polyclonal used at a 1/5000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue with ab52175.The picture on the right is treated with synthesized peptide.
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