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Synthetic peptide corresponding to Human NF-kB p65 aa 526-539.
This antibody binds to all forms of NF-KB p65, both activated (phosphorylated, dimers) and non-activated. NFkB in the nucleus is considered to be active.
Our Abpromise guarantee covers the use of ab13594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use 0.25-0.5µg for 106 cells.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian cystadenocarcinoma tissue labelling NF-kB p65 with ab13594 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Flow Cytometric analysis of NF-kB p65 in HEK 293 cells labelled with ab13594 at 0.5µg (Red), or isotype control antibody (green).
Western blot analysis of NF-kB p65 using ab13594 at 2µg/ml dilution against 10µg of Daudi cell lysate.
ab13594 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"