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Recombinant fragment corresponding to Human NF-kB p65 aa 432-505.
Our Abpromise guarantee covers the use of ab95020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.|
|ICC/IF||Use a concentration of 35 µg/ml.|
|Flow Cyt||Use 0.1µg for 106 cells. Ab81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab95020, at 35 µg/ml, staining NF-kB p65 in HeLa cell by Immunofluorescence.
Proximity Ligation Analysis (PLA) of protein-protein interactions between IKBKB and NF-kB p65. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal at 1:1200 and ab95020 at 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
ab95020 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"