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Our Abpromise guarantee covers the use of ab7970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent concentration.|
|ChIP/Chip||Use at an assay dependent concentration.|
|EMSA||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent dilution.|
|IHC-P||1/1000 - 1/4000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||1/400 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 69 kDa).Can be blocked with NF-kB p65 peptide (ab8005).|
|IHC-Fr||1/100. PubMed: 17003500|
|ICC/IF||Use at an assay dependent dilution. PubMed: 16818736|
|Flow Cyt||Use at an assay dependent concentration.|
ab7970 staining NF-kB p65 in Human AGS cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) for 1 hour at 25°C. An Alexa Fluor®568-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Western blotting starting dilution: 1/400-1/1000.
This image is a courtesy of Anonymous Abreview
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human DLBCL showing nuclear expression of NF-kB p65 in the tumor cells.
ab7970 staining NF-kB p65 in Human stomach tissue sections by IHC-Fr (Immunohistochemistry - Froxen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody 1/1000 in blocking buffer for 1 hour at 25°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.
ab7970 staining NF-kB p65 in mouse lung tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent xylene fixation before heat mediated antigen retrieval in citrate and then blocking with 1.5% serum was performed for 30 minutes at 37°C. The primary antibody was used diluted 1/500 and incubated with sample for 16 hours at 4°C. A Biotin conjugated polyclonal to rabbit IgG was used undiluted as secondary antibody. ABC staining system was used.
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