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Does not react withRat
Synthetic peptide corresponding to Human NF-kB p65 (C terminal).
Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.
Our Abpromise guarantee covers the use of ab32536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|IHC-P||Use at an assay dependent dilution.|
|ICC/IF||1/250 - 1/500.|
Image courtesy of an anonymous Abreview
Anti-NF-kB p65 [E379] antibody (ab32536) reactivity with reduced wild type (WT) and p65 knockout (KO) mouse embryonic fibroblast (MEF) lysate. After SDS-PAGE, membranes were blocked in 5% milk in TBS + 0.1% Tween for 1h at 25°C before incubation with ab32536 (1:1,000 dilution in 5% milk TBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
Immunohistochemical analysis of colon sections from mice, staining NF-kB p65 with ab32536.
Antigen retrieval was performed by microwave heating in citrate buffer, pH 6. Sections were incubated overnight with primary antibody (1/250) and staining was detected using ab80437 EXPOSE Rabbit specific HRP/DAB detection IHC kit.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"