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Synthetic peptide corresponding to Human NF-kB p65 aa 500-600 (C terminal).
Database link: Q04206
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A 40 µl trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab32536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/100. Ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
Image courtesy of an anonymous Abreview
Anti-NF-kB p65 [E379] antibody (ab32536) reactivity with reduced wild type (WT) and p65 knockout (KO) mouse embryonic fibroblast (MEF) lysate. After SDS-PAGE, membranes were blocked in 5% milk in TBS + 0.1% Tween for 1h at 25°C before incubation with ab32536 (1:1,000 dilution in 5% milk TBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
Immunohistochemical analysis of colon sections from mice, staining NF-kB p65 with ab32536.
Antigen retrieval was performed by microwave heating in citrate buffer, pH 6. Sections were incubated overnight with primary antibody (1/250) and staining was detected using ab80437 EXPOSE Rabbit specific HRP/DAB detection IHC kit.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"