Recombinant human NF2/ Merlin with His tag purified from E.coli
Our Abpromise guarantee covers the use of ab88957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
|Indirect ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Empty lane (40 µg)
Lane 3: NF2 / Merlin knockout HAP1 cell lysate (20 µg)
Lane 4: Empty lane (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab88957 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab88957 was shown to specifically react with NF2 / Merlin when NF2 / Merlin knockout samples were used. Wild-type and NF2 / Merlin knockout samples were subjected to SDS-PAGE. Ab88957 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) ab216772 and IRDye® 680 Goat anti-Rabbit IgG (H + L) ab216777 secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.