Anti-NF2 / Merlin (phospho S518) antibody (ab47378)

Overview

  • Product name
    Anti-NF2 / Merlin (phospho S518) antibody
    See all NF2 / Merlin primary antibodies
  • Description
    Rabbit polyclonal to NF2 / Merlin (phospho S518)
  • Specificity
    Detects endogenous levels of Merlin only when phosphorylated at serine 518.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Merlin around the phosphorylation site of serine 518 (R-L-SP-M-E)

  • Positive control
    • HuvEc cells

Properties

Applications

Our Abpromise guarantee covers the use of ab47378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ELISA 1/4000.

Target

  • Function
    Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
  • Tissue specificity
    Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
  • Involvement in disease
    Defects in NF2 are the cause of neurofibromatosis 2 (NF2) [MIM:101000]; also known as central neurofibromatosis. NF2 is a genetic disorder characterized by bilateral vestibular schwannomas (formerly called acoustic neuromas), schwannomas of other cranial and peripheral nerves, meningiomas, and ependymomas. It is inherited in an autosomal dominant fashion with full penetrance. Affected individuals generally develop symptoms of eighth-nerve dysfunction in early adulthood, including deafness and balance disorder. Although the tumors of NF2 are histologically benign, their anatomic location makes management difficult, and patients suffer great morbidity and mortality.
    Defects in NF2 are a cause of schwannomatosis (SCHWA) [MIM:162091]; also known as congenital cutaneous neurilemmomatosis. Schwannomas are benign tumors of the peripheral nerve sheath that usually occur singly in otherwise normal individuals. Multiple schwannomas in the same individual suggest an underlying tumor-predisposition syndrome. The most common such syndrome is NF2. The hallmark of NF2 is the development of bilateral vestibular-nerve schwannomas; but two-thirds or more of all NF2-affected individuals develop schwannomas in other locations, and dermal schwannomas may precede vestibular tumors in NF2-affected children. There have been several reports of individuals with multiple schwannomas who do not show evidence of vestibular schwannoma. Clinical report suggests that schwannomatosis is a clinical entity distinct from other forms of neurofibromatosis.
  • Sequence similarities
    Contains 1 FERM domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail.
    Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.
  • Cellular localization
    Cytoplasm > perinuclear region. Cytoplasmic granule. Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia; Cytoplasm > perinuclear region. Cytoplasmic granule. Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia; Nucleus. Cell projection > filopodium membrane. Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Cytoplasmic granule. Cytoplasm > cytoskeleton. In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia and Cell projection > filopodium membrane. Cell projection > ruffle membrane. Nucleus. In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACN antibody
    • BANF antibody
    • Bilateral acoustic neuroma antibody
    • MERL_HUMAN antibody
    • Merlin antibody
    • Moesin ezrin radixin like protein antibody
    • Moesin ezrin radizin like antibody
    • Moesin-ezrin-radixin-like protein antibody
    • Neurofibromatosis 2 antibody
    • Neurofibromatosis type 2 antibody
    • Neurofibromatosis2 antibody
    • Neurofibromin 2 antibody
    • Neurofibromin-2 antibody
    • Neurofibromin2 antibody
    • NF 2 antibody
    • Nf2 antibody
    • SCH antibody
    • Schwannomerlin antibody
    • Schwannomin antibody
    see all

Images

  • All lanes : Anti-NF2 / Merlin (phospho S518) antibody (ab47378) at 1/500 dilution

    Lane 1 : HuvEc cell extracts treated with IFN-a(1000U/ml, 18 hours)
    Lane 2 : HuvEc cell extracts


    Predicted band size : 69 kDa
    Observed band size : 69 kDa

References

This product has been referenced in:
  • Morrison H  et al. Merlin/neurofibromatosis type 2 suppresses growth by inhibiting the activation of Ras and Rac. Cancer Res 67:520-7 (2007). WB ; Human . Read more (PubMed: 17234759) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
30 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 03 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3 (fibroblast))
Specification
NIH3T3 (fibroblast)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton x-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Cui Yan

Verified customer

Submitted Dec 18 2007

Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma cell line and some other cell lines)
Loading amount
50000 cells
Specification
melanoma cell line and some other cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Cui Yan

Verified customer

Submitted Dec 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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