Overview

  • Product name
    Anti-Nfasc186 antibody - Neuronal Marker
  • Description
    Rabbit polyclonal to Nfasc186 - Neuronal Marker
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Rat Nfasc186.

    (Peptide available as ab32637.)

  • Positive control
    • Brain (Rat) Whole Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab31719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa).

Target

  • Relevance
    Nfasc186 (Neurofascin 186) is one of two alternatively spliced products of the Neurofascin (Nfasc) gene in rat; the other being Nfasc155. The establishment of paranodal axoglial junctions in myelinated nerves where voltage-gated sodium channels are concentrated at the nodes of Ranvier is determined by myelin-forming glia. The two isoforms of Neurofascin, Nfasc186 in neurons and Nfasc155 in glia, are required for the assembly of these specialized domains. These two major Neurofascins play essential roles in assembling the nodal and paranodal domains of myelinated axons and are essential for the transition to saltatory conduction in developing vertebrate nerves. Nfasc186 (Isoform 1) is expressed at the Nodes of Ranvier while the Nfasc155 (isoforms 2 and 3) are expressed in unmyelinated axons.
  • Cellular localization
    Cell Membrane; Type I membrane protein.
  • Database links
  • Alternative names
    • Neurofascin antibody
    • NF antibody
    • NF186 antibody
    • NFASC antibody
    • NRCAML antibody
    see all

Images

  • Anti-Nfasc186 antibody - Neuronal Marker (ab31719) at 1 µg/ml + Brain (Rat) Whole Tissue Lysate at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 186 kDa
    Observed band size : 186 kDa
  • Immunohistochemistical detection of Nfasc186 using antibody ab31719 on PFA perfusion-fixed rat  brain sections. Primary antibody was diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488® diluted (1/1000). Immunostaining observed (image) in the rat cortex. IHC was performed on free-floating sections using direct fluorescence. The antibody produced some staining in the whole CNS.

    See Abreview

References

This product has been referenced in:
  • Martin PM  et al. Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier. J Biol Chem 292:2441-2456 (2017). Read more (PubMed: 27979964) »
  • Brügger V  et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol 13:e1002258 (2015). Read more (PubMed: 26406915) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain cortex sections)
Specification
Brain cortex sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Mar 02 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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