Overview

  • Product nameAnti-NFAT1 antibody
    See all NFAT1 primary antibodies
  • Description
    Rabbit polyclonal to NFAT1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MDVPEPQPDP DGGDGPGHEP GGSPQDELDF SILFDYDYLN PIEEEPIAHK, corresponding to N terminal amino acids 1-50 of Human NFAT1

  • Positive control
    • SP2/0 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
  • Tissue specificityExpressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
  • Sequence similaritiesContains 1 RHD (Rel-like) domain.
  • DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • Post-translational
    modifications
    In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI607462 antibody
    • cytoplasmic 2 antibody
    • KIAA0611 antibody
    • NF ATc2 antibody
    • NF ATp antibody
    • NF-ATc2 antibody
    • NF-ATp antibody
    • NFAC2_HUMAN antibody
    • NFAT 1 antibody
    • NFAT pre existing subunit antibody
    • NFAT pre-existing subunit antibody
    • NFAT transcription complex, preexisting component antibody
    • NFAT1 antibody
    • NFAT1-D antibody
    • NFATc2 antibody
    • NFATp antibody
    • Nuclear factor of activated T cells cytoplasmic 2 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
    • Nuclear factor of activated T cells pre-existing component antibody
    • Nuclear factor of activated T cells, preexisting component antibody
    • Nuclear factor of activated T-cells antibody
    • Preexisting nuclear factor of activated T cells 2 antibody
    • T cell transcription factor NFAT 1 antibody
    • T-cell transcription factor NFAT1 antibody
    see all

Anti-NFAT1 antibody images

  • Anti-NFAT1 antibody (ab49161) at 1.25 µg/ml + SP2/0 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 100 kDa
    Observed band size : 100 kDa

References for Anti-NFAT1 antibody (ab49161)

This product has been referenced in:
  • Nagamoto-Combs K & Combs CK Microglial phenotype is regulated by activity of the transcription factor, NFAT (nuclear factor of activated T cells). J Neurosci 30:9641-6 (2010). WB ; Mouse . Read more (PubMed: 20631193) »
  • Heer R  et al. Phenotypic modulation of human urinary tract stroma-derived fibroblasts by transforming growth factor beta3. Urology 76:509.e13-20 (2010). WB ; Human . Read more (PubMed: 20546875) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"