Overview

  • Product name
  • Description
    Rabbit polyclonal to NFAT2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human NFAT2 (EAW66621.1).

  • Positive control
    • 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab124292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
  • Tissue specificity
    Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
  • Sequence similarities
    Contains 1 RHD (Rel-like) domain.
  • Domain
    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
    Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
  • Post-translational
    modifications
    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic 1 antibody
    • MGC138448 antibody
    • NF ATc antibody
    • NF ATc1 antibody
    • NF-ATc antibody
    • NF-ATc1 antibody
    • NF-ATc1.2 antibody
    • NFAC1_HUMAN antibody
    • NFAT 2 antibody
    • NFAT transcription complex cytosolic component antibody
    • NFATC 1 antibody
    • NFATc antibody
    • NFATc1 antibody
    • Nuclear factor of activated T cells cytoplasmic 1 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody
    • Nuclear factor of activated T cells cytosolic component 1 antibody
    • nuclear factor of activated T-cells 'c' antibody
    • Nuclear factor of activated T-cells antibody
    see all

Images

  • All lanes : Anti-NFAT2 antibody (ab124292) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 1 kDa


    Exposure time : 3 minutes
  • ab124292 at 1 µg/ml staining NFAT2 in 293T whole cell lysate immunoprecipitated using ab124292 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 101 kDa. Exposure time: 30 seconds

References

ab124292 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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